多胺在湖北光敏核不育水稻育性转换中的作用

THE ROLE OF POLYMINES IN THE FERTILITY TRANSFORMATION OF HPGMR

从红光、远红光处理下农垦58S叶、穗内源多胺的分析和多胺生物合成抑制剂的使用,对多胺在农量58S育性转换中的作用做了较为系统的研究。结果表明:农垦58S叶片多胺变化与育性转换无明显关系;幼穗的多按水平受光敏色素间接调控并与育性转换密切相关。在不同发育时期,光敏色素对多胺的调节作用不同,对育性起主要调节作用的多胺种类也可能不同;我胺对农垦58S育性转换的调节作用与多胺的含量及不同多胺间的比值有关,其中精胺(Spm)、亚精胺(Spd)含量和Spm/Spd比值可能是重要因素。多胺生物合成抑制剂甲基乙二醛—双脒腙(MGBG),能部分逆转红光诱导的不育性,并在长光照完全不育的条件下促进农垦58S产生少量可育花粉和自交结实。

By assaying endogenous polyamines (PAsl and the application of inhibitor of PAs biosynthesis, a systematic study on the role of PAs in the transformation of HPGMR Nongken 58S has been carried out. The result clearly showed that though obvious relation between the fertility transformation and the fluctuation of PAs was not observed in leaves, the level of PAs was not observed in leaves, the level of PAs in the young ears was closely correlated with the fertility transtormation. The fact that phytochrome has different functional effect at different stages of plant development along with the observation that the level of PAs in ears was regulated indirectly by phytochrome lead to the notion that different fractions of PAs might act in different stages of Nonken 58 development, including the fertility expression. Further study indicated that the content of spermidine (SPd) and spermine (SPIn) and the SPm/SPd ratio might play an important role in the fertility transformation of Nongken 58S. Also added to the above notion is another line of evidence that the effect of red light on Nongken 58S can be reversed partly by MGBG an inhibitor of PAs biosynthesis, and that MGBG can stimulate Nongken 58S to form fertile pollen and engender it self fertility under otherwise absolutely sterile conditions.