玉米CMS—C材料雄花育性不稳定性与DNA的关系

RELATIVITY BETWEEN MITOCHONDRIA DNA AND FERTILITY INSTABILITY OF CMS--S IN MAIZE

田间鉴定7个CMS材料雄花育性,发现Mo17CMS—ES、Mo17CMS—C、107CMS—ES、107 CMS—C、塘四10CMS—C 5个材料均为完全不育或高度不育株,77 CMS—C、关17CMS—C则分别出现了25—30%的半可育株。Mo17CMS—C、Mo17CMS—ES材料线粒体DNA经PstⅠ、SalⅠ酶切,与2.2kb探针杂交的电泳图谱与正常胞质Mo17的有差异;它们的总DNA经ECoRⅠ酶切,与2.2kb探针杂交,其电泳电谱与正常胞质Mo17的也有差异。研究结果说明核背景中的修饰基因与胞质亚组等位基因之间的互作共同控制CMS材料雄花育性的不稳定遗传的可能性。

Of the seven maize CMS lines assayed for pollen viability in the field, five (namely. MolTCMS--ES. MolTCMS--C. 107CMS--ES, 107CMS--C and Tangxu CMS--C) wet found completely sterile or highly sterile and two (namely 77CMS- C and Guan 17CMS--C) turned up 25--30%semi--fertile plants. Difference was also detected between Mol7CMS--C and Mo17CMS--ES and their normal counterpact Mo17 when mitochondria DNA and total DNA were subjected to hybridization with a 2.2kb plasmid-like probe following digestion by Pst Ⅰ and Sal Ⅰ, and EcoR Ⅰ respectively. It is thus speculated that muclear modifying gene and cytoplasm subgroup allele interact in controlling the instability of male fertility of CMS in maize.