光质和温度与2,4-D组合对喜树叶片脱分化的效应

THE EFFECT OF LIGHT QUALITY, TEMPERATURE AND 2,4-D ON DEDIFFERENTIATION OF BLADEIN CAMPTOTHECA ACUMINATA

喜树叶片在离体培养过程中,光质和温度与2,4-D组合对其脱分化亦有不同的效应。(1)当2,4-D浓度为0.5～1 mg/L时,不同光质诱导叶片脱分化率为:蓝光>黄光>绿光>黑暗>红光>自光;当2,4-D浓度为2～4 mg/L时,脱分化率为:蓝光和黄光>黑暗>绿光>白光>红光。(2)在不同的2,4-D浓度条件下,温度诱导叶片脱分化率为:25℃>20℃>30℃>35℃>10℃>4℃>40℃。(3)在同一光质或同一温度条件下。叶片脱分化率以2 mg/L2,4-D最佳,其次为4、1、0.5 mg/L。(4)蓝光有利于促进喜树叶片愈伤组织中可溶性蛋白质的合成,而红光有利于促进POD和SOD活性的提高。

(1) When the consistency of 2, 4-D was 0.5～1 mg/L, the dedifferentiation ratio of the blade in camptotheca acuminata under different light quality were in order of blue, yellow, green, dark, red and white light. When the consistency of 2, 4-D was 2～4 mg/L, the dedifferentiation ratio were in order of blue, yellow, dark, green, white and red light. (2) Under the different temperature, the dedifferentiation ratio were in order of 25℃, 20℃, 30℃, 35℃, 10℃, 4℃ and 40℃. (3) Under the same light quality or temperature, the dedifferentiation ratio with 2 mg/L 2, 4-D were the highest. (4) Protein contents of the callus was favourable increased by blue light, but POD and SOD activity of the callus were advanced by red light.