摘要
Western杂交是一种检测蛋白质的免疫化学方法,已在生物科学的许多领域得到广泛应用,但以前的文献介绍的Western杂交程序过于繁琐。本文根据Western杂交的基本原理结合已报道资料进行了一些改进,并应用到猪血清瘦素蛋白的检测。该程序简单易操作,结果清晰,证明改进后的方法完全可行。
Western blotting, an immunochemica! method for protein detection, has been employed in many biological fields,but the usual procedures are too cumbersome and need improvement. According to the basical principle of Western blotting and some methods of others,we improved the blotting procedure and practiced it in leptin boltting. It is simple and easy to handle,the results are clear. All proved to the method practical.
出处
《畜牧兽医杂志》
2003年第3期11-13,共3页
Journal of Animal Science and Veterinary Medicine
基金
西北农林科技大学专项课题经费资助
