菜薹茎尖培养中的激素与热激调控

Effect of Plant Growth Regulators and Heat Shock on Shoot-tip Culture in Brassica campestris L.ssp.chinensis var.utilis Tsen et Lee.

BA和NAA不同浓度组合对菜薹茎尖培养会产生不同的影响 ,BA 0 .8mg·L-1以上浓度能获得较好的增殖效果 ,NAA浓度不宜超过 0 .5mg·L-1。KT和ZT混用不及单一使用效果好。外植体放置方式会影响茎尖的增殖效率。 45℃ 2h以内热激处理可促进菜薹茎尖的增殖。

The propagation in vitro of shoot-tips and quality of plantlets of Brassica campestris L.ssp. chinensis var.utilis Tsen et Lee.were affected by the combination of BA and NAA with different concentrations.Higher rate of shoot proliferation with better quality of plantlets could be obtained when the concentration of BA in medium was more than 0.8mg·L -1 and optimal concentration of NAA was less than 0.5mg·L -1.The effect of KT or ZT respectively on propagation was better than that of the combination of the both when total concentrations were identical.The orientations of explants was found to affect the proliferation rate.Heat shock with shoot-tips at 45℃ for 2h could stimulate the proliferation of shoots.