Electrochemical Aptasensor Based on Prussian Blue-Chitosan-Glutaraldehyde for the Sensitive Determination of Tetracycline

In this paper, a novel and sensitive electrochemical aptasensor for detecting tetracycline(TET)with prussian blue(PB) as the label-free signal was fabricated. A PB-chitosan-glutaraldehyde(PB-CS-GA)system acting as the signal indicator was developed to improve the sensitivity of the electrochemical aptasensor.Firstly, the PB-CS-GA was fixed onto the glass carbon electrode surface. Then, colloidal gold nanoparticles(Au NPs) were droped onto the electrode to immobilize the anti-TET aptamer for preparation of the aptasensor.The stepwise assembly process of the aptasensor was characterized by cyclic voltammetry(C-V) and scanning electron microscope(SEM). The target TET captured onto the electrode induced the current response of the electrode due to the non-conducting biomoleculars. Under the optimum operating conditions, the response of differential pulse voltammetry(DPV) was used for detecting the concentration of TET. The proposed aptasensor showed a high sensitivity and a wide linear range of 109-～ 105-M and 105-～ 102-M with the correlation coefficients of 0.994 and 0.992, respectively. The detection limit was 3.2×1010-M(RSD 4.12%). Due to its rapidity, sensitivity and low cost, the proposed aptasensor could be used as a pre-scanning method in TET determination for the analysis of livestock products.

In this paper, a novel and sensitive electrochemical aptasensor for detecting tetracycline (TET) with prussian blue (PB) as the label-free signal was fabricated. A PB-chitosan-glutaraldehyde (PB-CS-GA) system acting as the signal indicator was developed to improve the sensitivity of the electrochemical aptasensor. Firstly, the PB-CS-GA was fixed onto the glass carbon electrode surface. Then, colloidal gold nanoparticles (AuNPs) were droped onto the electrode to immobilize the anti-TET aptamer for preparation of the aptasensor. The stepwise assembly process of the aptasensor was characterized by cyclic voltammetry (C-V) and scanning electron microscope (SEM). The target TET captured onto the electrode induced the current response of the electrode due to the non-conducting biomoleculars. Under the optimum operating conditions, the response of differential pulse voltammetry (DPV) was used for detecting the concentration of TET. The proposed aptasensor showed a high sensitivity and a wide linear range of 10(-9) similar to 10(-5) M and 10(-5) similar to 10(-2) M with the correlation coefficients of 0.994 and 0.992, respectively. The detection limit was 3.2x10(-10) M (RSD 4.12%). Due to its rapidity, sensitivity and low cost, the proposed aptasensor could be used as a pre-scanning method in TET determination for the analysis of livestock products.