血清抗S100B蛋白自身抗体的检测法及初步应用

Detection of anti-S100B autoantibodies in serum by ELISA

目的 建立一种检测血清抗S10 0B蛋白自身抗体的方法 ,探讨抗S10 0B自身抗体对精神及神经系统疾病的意义。方法用间接ELISA检测 112例健康人及 10 1例精神病及神经系统疾病患者血清抗S10 0B自身抗体。结果 建立的ELISA有较高的特异性。批内与批间平均变异系数分别为 5 6 %和 12 1%。抗S10 0B自身抗体阳性率 ,癫痫为 4 6 .6 7% (14 / 30 ) ,多发性硬化为4 5 .83% (11/ 2 4 ) ,帕金森病为 6 3.6 4 % (14 / 2 2 ) ,抑郁症为 4 0 .0 % (12 / 2 5 )。结论 该法可有效地检测抗S10 0B自身抗体 ;某些精神病患者或神经系统疾病患者血清中抗S10

Objective To establish a method for detection of serum anti S100B autoantibodies and explore the significance of IgG class anti S100B autoantibodies in patients with mental and neurological disorders.Methods An ELISA was developed to quantify anti S100B autoantibodies levels in the sera of 112 normal individuals and 101 patients.Results The IgG class natural autoantibodies to proteins S100B were presented in the serum of healthy adults.The percentages of increasing anti S100B autoantibodies of serum levels in various diseases were as follows:46.7%(14/30) for epilepsy 46.67% was found,that the 45.8%(11/24) for multiple sclerosis,63.6%(14/22) for Parkinson's disease and 40.0%(12/25) for depressive disorder.Conclusion The developed method in this study is effective for the detection of the serum levels of anti S100B autoantibodies which increase may associated with autoimmunity of central nervous system.