功率超声波在马铃薯渣酶解工艺中的应用

Application of Power Ultrasonic in Enzymatic Hydrolysis Process of Potato Pulp

利用超声波预处理半纤维素酶,使其活性得以提高,进而提高马铃薯渣的酶解效率。首先,考查酶解条件,即pH值、水浴温度和水浴时间对马铃薯渣酶解效果的影响;其次,考查了超声波预处理条件(如超声功率、超声处理时间)对半纤维素酶活性的影响;再次,考查了放置时间(0~2.5 h)对超声处理后的半纤维素酶活性的影响。结果表明,pH值5.0~6.0,水浴温度50℃,水浴时间15 min为半纤维素酶的最佳酶解条件;超声功率600 W,超声时间4 min为半纤维素酶的最佳超声处理条件;在放置时间为0~2.5 h时,超声波处理后的半纤维素酶与未处理的酶溶液相比,依然保持了较高的酶活性(p<0.05)。该研究表明,超声波预处理有助于提高半纤维素酶活性。

Inor order to increase the hemicellulase activity with power ultrasonic pretreatment,and then to improve the enzymatic hydrolysis efficiency of potato pulp. First,the effect of enzymatic hydrolysis conditions were evaluated such as pH value,water bath temperature and water bath heating time on the enzymatic hydrolysis effectiveness of the potato pulp. The secondary,the effect of ultrasonic pretreatment conditions such as ultrasonic power and ultrasonic treatment time on the hemicellulase activity was evaluated. Moreover,the effect of storage time(0~2.5 h) on the hemicellulase activity of the ultrasonic treated sample was also evaluated. Result indicated that the optimal enzymatic hydrolysis conditions for hemicellulase activity were pH 5.0~6.0,water bath temperature 50 ℃, water bath heating time 15 min. The optimal conditions for ultrasonic pretreatment of the hemicellulase were ultrasonic power 600 W,ultrasonic time 4 min. Between the 0~2.5 h of storage time,the ultrasonic treated sample was still has higher hemicellulase activity compared with the un-ultrasonic treated sample(p<0.05). This study indicated that the power ultrasonic pretreatment was useful for increase of hemicellulase activity.