黄芪对脑缺血再灌注星形胶质细胞的影响

Effects of Radix astragali on Astrocytes After Cerebral Ischemia and Reperfusion

目的 :为了探讨黄芪对脑缺血再灌注后星形胶质细胞 (AS)的影响。方法 :采用沙土鼠双侧颈动脉夹闭脑缺血模型 ,于脑缺血 15min再灌注 2 4h和 4 8h后运用免疫组织化学技术观察星型胶质纤维酸性蛋白 (GFAP)的动态表达。结果 :缺血 15min再灌注 2 4h后 ,GFAP免疫阳性反应达高峰 ,黄芪可使GFAP免疫反应减轻 ;缺血 15min4 8h后GFAP表达减弱 ,黄芪可使其增强。结论 :黄芪对脑缺血再灌注后星形胶质细胞的调节作用可能与脑缺血损伤后神经功能的恢复有关。

Objective To study the effects of Radix on astrocytes after cerebral ischemia and reperfusion.Methods Cerebral ischemia model in gerbils was produced by Ligating bilateral common carotid artery.The dynamic expressin of GFAP were determined by immunochemistry after cerebyal ischemia for 15min followed by reperfusion for 24 hours and 48 hours.Result GFAP positive reactions reached a peak after cerebral ischemia for 15 min followed by reperfusion for 24 hours. Radix decreased the expression.GFAP positive reactions decreased after cerebral ischemia for 15 min followed by reperfusion for 48 hours,Radix increased the expression.Conclusion The regulation of Radix on astrocytes after cerebral ischemia and reperfusion may be related with repairing process after cerebral ischemia.