转化生长因子β_1在糖尿病大鼠心肌中的表达及福辛普利的干预

The expression of TGF-P, in myocardium of diabetic rats and the effect of fosinopril

目的 研究转化生长因子β_1(TGF-β_1)在糖尿病大鼠心肌中的表达及福辛普利(Fosino-pril)的干预作用。方法 大鼠20只建立糖尿病模型后,随机分为福辛普利治疗组及糖尿病未治疗组,每组10只,用免疫组织化学法检测糖尿病大鼠心肌TGF-β_1的表达及用福辛普利治疗后的变化,并与正常组对照。结果 正常组心肌细胞胞浆内,TGF-β_1表达呈弱阳性反应;糖尿病未治疗组呈强阳性反应;福辛普利治疗组呈阳性反应。用图像分析仪计算其平均吸光度A值分别为0.009±0.004、0.198±0.007及0.104±0.005。未治疗组TGF-β_1的表达明显高于正常组及治疗组(P<0.01)。结论 TGF-β_1在糖尿病大鼠心肌中的表达明显增强,而福辛普利能在一定程度上抑制其表达从而可能延缓糖尿病性心肌病的病理学进展。

Objective To investigate the effect of fosinopril on transforming growth factor-β1 (TGF-β1) expression in myocardial tissue of streptozotocin(STZ)-induced diatetic rats. Methods A total of 30 SD rats were enrolled in the study, 10 of them were set as normal controls, others were created the model of diabetes by injecting STZ, and then equally divided into fosinopril group and untreated group. Myocardial TGF-ft was observed in all three groups after 5 weeks. Results The expression of TGF-fi, in myocardial tissue was weakly positive in normal group, strongly positive in untreated group and positive in fosinopriol treat group, with absorbance being 0. 009 ± 0. 004,0. 198 ±0. 007 and 0. 104 ± 0. 005,respectively. Conclusion Fosinopril may partly suppress the increased TGF-β1 expression in myocardial tissue of diatetic rats. It may decrease significantly the myocardial injury of diabetic rats.