小鼠肾脏自溶过程中超微结构的动态变化

Ultrastructure changes of kidney autolysis at different time points in mice

目的 :观察死亡小鼠在不同时间内肾脏自溶的超微结构变化。方法 :采用透射电镜技术 ,观察小鼠死后0h、5h、10h、2 0h、4 0h肾皮质超微结构的改变。结果 :随死亡时间的延长 ,小鼠肾小管上皮细胞出现一系列自溶改变。主要表现为微绒毛和溶酶体的逐渐消失 ,线粒体变性 ,核染色质边集 ,髓鞘样结构出现并增多 ,核周隙增宽及裸核的出现。结论

Aim: To investigate the ultrastructure changes of kidney autolysis at different time points in postmortem mice. Methods:The dynamic ultrastructural changes of the renal cortex were observed with transmissional electron microscope in mice at 0,5,10,20 and 40 h after death, respectively. Results: Series of autolytic changes were found in the epithelial cells of the renal tubes as the time was prolonged. These changes included that the microvilli and lysosome disappeared gradually,the mitochondria degenerated, the chromatin of nucleus collected aside, the myeline figures appeared and increased, the perinucleus space widened,and the nude nucleus appeared. Conclusion: The ultrastructure changes of kidney can be used to determine the death time for rats.