反相离子对色谱法测定盐酸吗啉双胍的含量

RP-HPLC assay for the determination of moroxydini hydrochloridi in compound metamizole sodium injection

目的 :建立一种新的测定吗啉双胍含量的方法。方法 :对样品进行衍生化后 ,采用反相离子对色谱法(RP HPLC)分离测定。色谱条件为C18色谱柱 (2 5 0mm× 4 .6mm ,5 μm) ,0 .0 2mol·L-1辛烷磺酸钠为离子对试剂 ,水 冰醋酸 甲醇 (体积比为 30 5 6 5 )为流动相 ,流速为 1.1ml·min-1,检测波长为 330nm。结果 :在 0 .2 0～ 0 .6 2g·L-1浓度范围内线性关系良好 ,r=0 .9996 (P <0 .0 5 ) ,平均回收率 10 0 .13% ,RSD为 0 .6 %。结论 :本法简便、快速、准确 。

Aim:To develop a new RP HPLC method for the determination of moroxydini hydrochloridi. Methods: Chromatographed condition: C 18 column(250 mm×4.6 mm, 5 μm); 0.02 mol·L -1 nacalai tesque was ion pair, the mobile phase was H 2O HAc MeOH(30565),and detection wave length was 330 nm.Results: There was a good linear relationship over the range of 0.20～0.62 g·L -1 , r =0.999 6.The average recovery was 100.13% with RSD 0.6%( n =5). Conclusion:The method was simple, rapid, accurate and can be used to assay moroxydini hydrochloridi.