针药结合对去势大鼠血中性激素及其受体作用的实验研究

Effects of acupuncture combined with medicine on serum sexual hormones and their receptors in castrated rats

目的:探讨针药结合对去势大鼠血清雌二醇(E_2)、卵泡刺激素(FSH)、黄体生成素(LH)及外周血淋巴细胞内雌激素受体(ER)水平的影响。方法:以雌性成年未孕SD大鼠为对象,设更年Ⅱ号组、针刺组、针药结合组、更年康对照组、模型对照组和正常对照组,测定血清E_2、FSH、LH的含量,及外周血淋巴细胞内ER的含量。结果:(1)去势大鼠血清E_2水平明显低于对照组,FSH和LH水平明显高于对照组。治疗后各组大鼠E_2水平与模型对照组比较均明显升高,FSH和LH水平明显下降,但以针药结合组作用最为显著。(2)去势后大鼠的外周血淋巴细胞ER的含量比对照组明显下降,经针刺或更年Ⅱ号及针药结合治疗后3组大鼠淋巴细胞ER含量与模型对照组比较明显上升,但以针药结合组作用最为明显,与更年Ⅱ号和针刺组比较差异有显著性(P<0.01)。更年康对照组对淋巴细胞ER无明显影响。结论:针刺和更年Ⅱ号不仅能提高去势大鼠血清E_2水平,降低促性腺激素LH、FSH水平,而且可以上调免疫细胞ER的含量,而针药结合组作用最为明显。

Objective: To explore effects of acupuncture combined with medicine on serum E2. FSH, LH and ER level in peripheral blood lymphocytes. Methods: Female SD rats without pregnancy were divided into Gengnian I group, acupuncture group, group of combined acupuncture and medicine, Gennian Kang control group, model control group and normal control group, and their serum level of E2, FSH and LH contents, and ER contents in peripheral blood lymphocytes were determined. Results: (1) Serum E2 level in castrated rats was lower than, and FSH and LH level were higher than those in the control group. E2 level in all other groups increased, and FSH and LH level decreased significantly after treatment as compared with those in model control group, with the most obvious action in the group of combined acupuncture and medicine. (2) ER content in castrated rat decreased significantly as compared with control group. After treatment of acupuncture. Gengnian I or acupuncture combined with medicine. ER contents increased significantly as compared with that in model control group, with the most obvious action in the group of combined acupuncture and medicine. Gengnian Kang had no remarkable influence on ER. Conclusion: Acupuncture and Gengnian I can increase serum E2 level, decrease FSH and LH level, and up-regulate ER content in immunocytes with the most obvious action for the combined treatment of acupuncture and medicine.