超声提取法测定魔芋精粉葡甘露聚糖含量的实验研究

Studies on Ultrasonic Wave Extracting Method Determining Konjac Glucomannanin Konjac Refined Powder

目的 :探索超声波处理对提取魔芋精粉中葡甘露聚糖 (konjacglucomannan ,KGM )的影响。 方法 :用超声波清洗器除去魔芋精粉中的游离还原糖后超声水提KGM ,分光光度法测定水解液中KGM含量。结果 :固定超声波频率 40kHz,功率 1 0 0W时 ,超声水提 30～ 45min ,KGM含量与常规法相当 ;超声水提 1h ,KGM含量明显高于常规水提 4h(P <0 .0 1 ) ,提高率为 6 .5 %。本法标准葡萄糖量在 0 .2～ 1 .6mg线性关系良好 (r =0 .9996) ,加样平均回收率为 97.8% ,重现性为 1 .2 7%。结论 :超声水提法省时、可靠 ,能提高魔芋精粉中KGM的提取效率。

Objective:To investigate the effect of ultrasonic wave on extracting Konjac Glucomannan(KGM)in Konjac refined powder. Method:Free reduced sugar in Konjac refined powder was removed and Konjac refined powder in the aqueous solution was processed by ultrasonic wave and KGM content was measured by spectrophotometry. Result:KGM content in the Konjac refined powder aqueous solution by ultrasonic process at fixed 40 kHz,100 W, 30 45 min was equal to that by routine method at 4 h; whereas, by 1 h of ultrasonic process, KGM content was significantly enhanced than that by 4 h of routine method( P <0.01),enhancement rate was 6.5%. Linearity of standard glucose was good ( r =0.999 6) in range of 0.2 1.6 mg.The average recovery was 97.8%, RSD of repeatability was 1.27%. Conclusion:Ultrasonic extraction in aqueous solution is a reliable and rapid method that can enhance extraction efficiency of KGM in Konjac refined powder.[