熟地黄对毁损下丘脑弓状核大鼠学习记忆及海马c-fos,NGF表达的影响

Effect of Shu Di-huang on the Expression of c-fos and NGF in Hippocampi and Learning and Memory of Rats Damaged Thalamic Arcuate Nucleus

目的 :研究熟地黄改善学习记忆的作用及机理。方法 :采用谷氨酸单钠 (MSG )毁损下丘脑弓状核大鼠模型 ,通过跳台实验和Morris水迷宫实验观察熟地黄对学习记忆的影响 ;免疫组化法观察大鼠海马c fos ,神经生长因子 (NGF)表达。结果 :熟地黄能延长MSG大鼠跳台实验潜伏期、减少错误次数 ;缩短水迷宫实验寻台时间 ,提高垮台百分率 ;提高c fos,NGF在海马的表达。结论 :熟地黄有改善MSG大鼠学习记忆作用 ,作用机理可能与提高c fos。

Objective: To study the mechanism of Shu Di huang in improving the function of learning and memory. Method: On the rats model with thalamic arcuate nucleus dameged by MSG, the improving function of Shu Di huang on learning and memory was observed by step down task and Morris water maze task, and the expression of c fos and NGF in hippocampi was observed by immunohistochemical means. Result: Shu Di huang could decrease the times of mistakes and prolong the incubation period in step down task, and shorten the incubation period of seeking the platform, and improve the percentage rate through the platform position in Morris water maze task. Shu Di huang also increase the expression of hippocampal NGF, c fos. Conclusion: Shu Di huang can improve the function of learning and memory of MSG rats,and its mechanism may be related with the increase of the expression of hippocampal c fos and NGF. [