丹龙醒脑片对沙土鼠脑缺血再灌注保护作用的实验研究

An Experimental Study on the Effects of DLXNP on the Gerbils Models of Cerebral Ischemia and Reperfusion

目的:研究丹龙醒脑片(DLXNP)对脑缺血再灌注沙土鼠模型脑组织兴奋性氨基酸(EAAs)含量的影响及对海马区神经元的保护作用。方法:用双侧颈总动脉反复夹闭再灌注方法建立脑缺血再灌注沙土鼠模型。用高效液相色谱分析荧光法(HPLC)检测脑组织谷氨酸(Glu)、天门冬氨酸(Asp)浓度,用病理光学显微镜观测其双侧海马CA_1区神经元数目。结果:模型组与正常对照组和假手术组相比,Glu、Asp含量明显升高(P<0.01),海马区神经细胞数明显减少(P<0.01)。丹龙醒脑片3个剂量组脑组织中Glu、Asp的含量明显低于模型组(P<0.01～0.05),海马区神经细胞数明显多于模型组(P<0.01)。结论:丹龙醒脑片能调节缺血再灌注大鼠脑组织兴奋性氨基酸含量、减轻其兴奋性毒性,保护大脑海马区神经元,这可能是丹龙醒脑片的作用机制之一。

Objective: To investigate the intervenient effects of DLXNP on cerebral EAAs and its protection effects on hippocampal cells following cerebral ischemia and reperfusion in the gerbils models. Methods : The ischemia and reperfusion model was made by repeatedly occludding bilateral carotid arteries (CCA) with artery clips for two times, every time for 5 minutes, brains were recirculated for 10 minutes by removal of clips between the two times and after the last occludding. Samples were extracted from hippocampal region and then made paraffin section and HE staining so as to make observation on hippocampal cell pathomorphology, hippocampal cells number was counted. Cerebral EAAs were detected by HPLC for the samples extracted from cerebral tissues. Results : After cerebral ischemia reperfusion, compared with the normal group and sham operation group, the model group's hippocampal cells number decreased (P<0.01), the concentrations of Glu, Asp increased (P<0.01). Compared with the modeling group, the contents of Glu, Asp of the three dosed DLXNP groups decreased (P<0.01-0.05), the number of three dosed DLXNP groups' hippocampal cells is more than those of the model group (P<0.01), the therapeutic effects of the middle dosed and the high dosed DLXNP are similar to that of the nimodipine (P>0.05). Conclusion : After the cerebral ischemia reperfusion, excitatory neurotoxicity of EAAs improved, DLXNP has the function of regulating EAAs and relieving its excitatory nerotoxicity, which may be one of the mechanisms of the actions of DLXNP.