高效液相色谱法测定肾炎康片中大黄酚的含量

HPLC Determination of Chrysophanol in Shenyangkang Tablets

目的:建立肾炎康片中大黄酚的含量测定方法。方法:采用高效液相色谱法,以十八烷基键合硅胶为填料,甲醇-0.025mol/L磷酸(80:20)为流动相,检测波长为428nm。结果:大黄酚与相邻组分分离度良好,回收率为95.5％,RSD为1.7％。结论:本法简便、准确、重现性好,可用于该制剂的质量控制。

Objective : To establish a HPLC methods for determination of the content of chrysophanol in Shenyankang Tablets. Methods: The packing in HPLC was stearyl bonded silica gel and mobile phase was consisted of methanol and phosphoric acid (0.025mol/L) as 80 to 20 parts with the determined wave length of 428nm. Results : The resolution of chrysophanol with other constituents in the system showed much better. The average recovery was 97.5% and RSD was 1.7%. Conclusion: Our method was simple, accurate and reproducible to be suitable for the quality control of the drug.