聚合酶链反应分析急性视网膜坏死的致病病毒

Polymerase Chain Reaction-based Analysis of the Viral Causes of Acute Retinal Necrosis Syndrome

目的研究聚合酶链反应分析急性视网膜坏死综合征眼内液病毒DNA的临床价值。方法聚合酶链反应检测28例37个眼内液标本疤疹病毒DNA,分析检测结果、影响因素及最终诊断。结果发现水痘-带状疱疹病毒DNA 15例,单纯疱疹病毒1型DNA 3例。适宜检测时间为炎症活动期,不用或短期使用抗病毒药。前房水和玻璃体均可取得可靠结果。结论聚合酶链反应检测眼内液病毒DNA,方法简便,尤其有助于疑难病例的诊断。

Purpose To investigate the clinical value of polymerase chain reaction (PCR) detecting viral DNA in acute retinal necrosis syndrome. Methods PCR was performed on 28 cases (37 intraocular fluid samples). PCR result, influence factors and final diagnosis were analyzed. Results It was identified that 15 cases were varicella zoster virus DNA and 3 cases were herpes simplex virus-1 DNA. Suitable time for PCR was during the active phase without or with short time use of antiviral therapy. Both aquenous humour and vitreous samples can contribute to the reliable results. Conclusion PCR is an easy method to make the raliable identification of the viral cause in ARN syndrome, especially in the clinical diagnostic dilemmas.