大肠肿瘤COX-2的表达及与细胞增殖的关系

Expression of cyclooxygenase-2 in human colonic tumors and its relationship to cell proliferation

目的 检测环氧化酶 (cyclooxygenase ,COX)及增殖细胞核抗原 (proliferatingcellnuclearanti gen ,PCNA)蛋白在大肠正常粘膜、腺瘤和癌组织中的表达 ,探讨COX 2在大肠肿瘤发生发展中的意义及其与细胞增殖的关系。方法 采用链霉素抗生物素蛋白 生物素 过氧化物酶免疫组化染色方法。结果 三组间的差异有显著性意义 (P <0 .0 1 ) ,大肠腺瘤PCNA表达在COX 2阳性者显著高于阴性者 (P <0 .0 5 ) ,但在大肠癌中 ,PCNA表达在COX 2阳性及阴性中无显著性差异 (P >0 .0 5 )。结论 COX 2的表达在大肠正常粘膜、腺瘤、腺癌中有逐渐上调趋势。在大肠腺瘤中 ,COX

Objective To detect the expression of cyclooxygenase 2 (COX 2) in human colonic adenomas and cancers, and its relationship to cellular proliferation.Methods Immunohistochemistry was performed by using avidin biotin peroxidase complex procedure.Results There was significantly difference of proliferating cell nuclear antigen (PCNA) between COX(+) and COX(-) in adenomas but not in cancers.Conclusion COX 2 expression was gradually up regulated in the sequence of normal epithelium, adenomas and adenocarcinomas. In adenomas, the expression of COX 2 was correlated with PCNA.