人类白细胞抗原DRB1等位基因与乙肝后肝硬化遗传易感性的研究

The association of HLA-DRB1 allele polymorphism with the genetic susceptibility to liver cirrhosis due to hepatitis B virus

目的 研究人类白细胞抗原 ( human leucocyte antigen,HL A) - DRB1等位基因多态性与湖北汉族乙肝后肝硬化遗传异感性的关系 ,为寻找乙肝后肝硬化的易感基因或抗病基因提供线索。方法 应用聚合酶链反应 -序列特异性引物技术对 10 6例湖北汉族乙肝后肝硬化患者和 10 8名正常健康对照者进行了 HL A- DRB1等位基因检测 ,并结合临床资料进行比较分析。结果 乙肝后肝硬化组 HL A- DRB1* 12 0 1/12 0 2等位基因频率明显升高 ( 2 0 .2 8% vs6 .0 1% ,RR=4 .9878,P<0 .0 0 1) ,HL A- DRB1* 15 0 1/ 15 0 2等位基因频率明显下降 ( 16 .6 7% vs6 .6 % ,RR=0 .30 4 3,P<0 .0 5 ) ,其他等位基因在两组之间差异无显著性。结论 HL A- DRB1* 12 0 1/ 12 0 2等位基因可能是湖北地区汉族人群乙肝后肝硬化的易感基因 ,HL A- DRB1* 15 0 1/ 15 0

Objective To assess the association of human leucocyte antigen (HLA) DRB1 allele with the genetic susceptibility to cirrhosis due to hepatitis B virus(HBV). Methods One hundred and six patients with cirrhosis due to HBV in Hubei area were investigated for HLA DRB1 gene by polymerase chain reaction sequence specific primers technique. The results were compared with those from 108 normal healthy people. Results The frequency of HLA DRB1*1201/1202 allele was 20.28% in patient group, which was significantly higher than the frequency (6 01%) in control group, the relative risk (RR) being 4.9878 ( P <0.01). The frequency of HLA DRB1*1501/1502 allele was decreased in patient group (patient 6.6%, control 16.67%, RR=0.3043, P <0 05), while the frequencies of other HLA DRB1 alleles were not significantly different( P >0.05). Conclusion HLA DRB1*1201/1202 allele may be the susceptibility gene in patients with cirrhosis due to HBV in Hubei Han nationality; HLA DRB1*1501/1502 allele is a resistant gene in patients with cirrhosis.