马卡小麦耐湿性的RAPD标记及定位研究

Genetic mapping of genes conferring waterlogging-tolerance in Triticum macha using RAPD markers

以一个耐湿的马卡小麦 (TriticummachaDek .etMen ,普通小麦的变种 )种质 (编号 4 9)和不耐湿的小麦宁麦3号为亲本 ,用一粒传法构建成由 115个F6代重组自交系组成的遗传群体。对亲本及其重组自交系的耐湿性鉴定表明 ,群体的耐湿性接近主峰分布。选择极端耐湿和不耐湿的重组自交系组成两个池 ,利用 16 4个RAPD引物对其进行多态性分析 ,发现引物S12 4 9在亲本和池间均检测出多态性。群体分析表明 ,该多态性位点与耐湿性呈极显著相关 ,它所解释的耐湿性表型变异为 13 9%。用中国春缺体四体初步确定 ,与马卡小麦耐湿性相关的这个位点定位在 5A染色体上。

In order to identify DNA markers for genes conferring waterlogging tolerance in Triticum macha Dek. et Men,115 F 6 recombinant inbred lines(RIL)were developed from a cross between Triticum macha accession 49(tolerant to waterlogging)and Ningmai 3(susceptible to waterlogging)using single seed descendant method.Among the RIL,the variation of waterlogging tolerance,represented by the ratio of the green leaf number of the treated to the untreated at booting stage,displayed a two peak distribution.Among 164 RAPD primers used in screening polymorphic markers,S1249 detected a polymorphic locus between the two parents and between tolerant and susceptible bulks.This locus showed significant correlation to waterlogging tolerance of the population,explaining 13 9% of total phenotypic variation.This marker was tentatively assigned to chromosome 5A of wheat using the nulli tetra lines of Chinese Spring.