miR-363靶基因预测及生物信息学分析

Target genes prediction and bioinformatics analysis of hsa-miR-363

目的 :初步明确hsa-miR-363潜在的生物学功能。方法:首先通过miRbase、UCSC等在线数据库对hsa-miR-363的碱基序列、基因位点及序列保守性进行分析;其次选择miranda、miRDB、Pic Tar及Target Scan四个在线数据库预测hsa-miR-363的靶基因并取交集,筛选后的靶基因用于后续分析;最后通过基因GO功能注释、富集分析和KEGG信号转导通路富集分析,初步阐明hsa-miR-363靶基因参与调控的细胞功能与信号通路。结果:根据生物信息学分析的结果显示,hsa-miR-363的功能较广泛,其多个潜在靶基因均参与子宫内膜异位症发生、发展过程中的多个生物学进程。结论:hsa-miR-363很可能与子宫内膜异位症的发病机制密切相关,并有可能为临床的靶向治疗提供潜在的新靶点。

Objective: To identify the potential biological function of hsa-miR-363. Methods: Firstly we analyzed hsa-miR-363 base sequence,chromosomalposition and conservation of the sequence through miRbase and UCSC databases. Secondly, we predicted the target genes by the miRNA databases, including Miranda, miRDB, Pic Tar, and Target Scan. Then the intersection of these predicted genes were done for further study. Finally, we clarified those cell functions and signaling pathways regulated by hsa-miR-363 target genes using GO and KEGG pathway analysis. Results: According to these bioinformatics data, we found that hsa-miR-363 might have a wide range of functions, and some potential target genes of this micro RNA participated in the initiation and development of endometriosis. Conclusion: Hsa-miR-363 might be related to the pathogenesis of endometriosis, and it may provide a potential target for the clinical therapy to endometriosis.