miR-497抑制肝癌细胞的侵袭与转移

miR-497 reduces invasion and migration of hepatocellular carcinoma

目的 :检测miR-497/IGF-1R在肝癌中的表达并探讨其在肝癌侵袭与转移中的作用。方法 :定量PCR(real-time PCR)检测肝癌及癌旁组织中miR-497的表达,同时检测肝癌细胞系及正常人肝细胞中miR-497/IGF-1RmRNA的表达水平,免疫组织化学及Western blot检测肝癌与肝癌细胞系中IGF-1R蛋白表达,通过过表达或干扰肝癌细胞系中miR-497的表达,分析其对肝癌细胞侵袭与转移的作用。结果:肝癌组织中miR-497表达水平低于癌旁组织。与无脉管转移组相比,有脉管转移组降低更为明显。同时在肝癌细胞系也有相似的结果,高侵袭性肝癌细胞系MHCC-97H中降低最为明显。IGF-1R在肝癌组织及肝癌细胞系中表达增加。过表达miR-497能降低MHCC-97H中IGF-1R的表达,抑制其侵袭和转移;干扰miR-497表达能增加IGF-1R表达,促进SMMC-7721细胞的侵袭和转移。结论 :miR-497在肝癌组织中表达降低,过表达miR-497能下调IGF-1R,抑制肝癌的侵袭与转移,miR-497可能为治疗肝细胞肝癌提供新的靶点。

Objective:To detect the expression and explore the role of miR-497 / IGF-1R in hepatocelluar carcinoma(HCC)invasion and migration. Methods: The expressions of miR-497 / IGF-1R in HCC samples and cell lines were detected by real-time PCR. Meanwhile, IGF-1R protein in HCC tissues and cell lines was assessed by immunohistochemistry and Western blot. In addition, the invasion and migration of HCC cells were analyzed by overexpressing or suppressing miR-497. Results: The expression of miR-497 was decreased in HCC samples compared with the adjacent non-cancer tissues, especially in HCC samples with vascular metastasis. The same results were observed in HCC cell line, the expression level of miR-497 decreased much more significantly in MHCC-97 H than other cell lines. IGF-1R expression was significantly increased in HCC and HCC-derived cells. Mi R-497 overexpression decreased the expression of IGF-1R and suppressed the invasion and migration of MHCC-97 H. Mi R-497 silenced SMMC-7721 cellular invasion and migration, which were enhanced with a boost in IGF-1R. Conclusion: This study indicates that miR-497 is downregulated in hepatocellular carcinoma, and miR-497 overexpression suppresses tumor cell invasion and migration with a decreased expression of IGF-1R. The study of miR-497 may provide a new therapeutic option for HCC.