ICR小鼠卵母细胞通过旁分泌机制促进排卵关键过程

Oocytes control of key ovulatory processes in ICR mice

目的:利用国内医学研究常用ICR品系小鼠,在构建用于研究卵母细胞-卵丘细胞交互作用培养模型的基础上,研究卵母细胞及其产生的旁分泌因子生长分化因子9(GDF9)和骨形态发生蛋白15(BMP15)对排卵过程中的关键事件——卵丘扩展的调控作用。方法:通过显微手术制备摘除卵母细胞后的卵丘细胞复合体(OOX),并通过与卵母细胞共培养或GDF9和BMP15处理研究卵母细胞及其特异释放的旁分泌因子对表皮生长因子(EGF)诱导卵丘扩展过程的调控。结果 :成功构建了ICR小鼠OOX培养模型,发现卵母细胞及其释放的旁分泌因子GDF9和BMP15为EGF诱导卵丘扩展所必需。结论:ICR小鼠卵母细胞通过旁分泌机制促进排卵关键过程。

Objective:To establish a culture model for studying the interaction between oocytes and cumulus cells in the strain of ICR mice that are frequently used in biomedical research in China,and using this model to study the effect of oocytes and oocytederived paracrine factors growth differentiation factor 9(GDF9) and bone morphogenetic protein 15(BMP15) on cumulus expansion,which is one of the key ovulatory processes. Methods:Oocytectomized cumulus complexes(OOX)were constructed by microsurgical removal of the oocyte from the cumulus-oocyte complexes,and the effect of oocyte and paracrine factors on EGF-induced cumulus expansion was studied by co-culturing the OOX with oocytes and treating the OOX with recombinant GDF9 and BMP15. Results:The model of OOX culture was successfully established using ICR mice,and the oocyte and its paracrine factor GDF9 and BMP15 were found to be essential for EGF to induce cumulus expansion. Conclusion:Oocytes promote key ovulatory processes in ICR mice.