肾母细胞瘤过表达基因促进肝癌细胞的侵袭与转移

Nephroblastoma overexpressed gene promotes migration and invasion of hepatocellular carcinoma

目的:探讨肾母细胞瘤过表达基因(NOV CCN3)在肝癌中的表达及在肝癌侵袭中的作用。方法 :荧光定量PCR(realtime PCR)检测肝癌及癌旁组织中的CCN3的m RNA表达水平,同时检测肝癌细胞系及正常人肝细胞中CCN3的m RNA表达水平,Western blot检测肝癌及癌旁组织中CCN3蛋白的表达量;通过肝癌细胞过表达CCN3分析CCN3蛋白对肝癌细胞转移的作用;运用Transwell侵袭实验和划痕迁移实验研究CCN3过表达对肝癌细胞侵袭和迁移能力的影响。结果:50对样本中有39对CCN3的m RNA表达水平在肝癌组织中高于癌旁组织,Western blot提示肝癌组织中CCN3蛋白表达量高于癌旁组织。体外肝癌细胞的功能试验中,过表达CCN3能促进MHCC-97H、SMMC-7721细胞的侵袭与转移。结论:CCN3在肝癌组织中高表达,过表达CCN3能促进肝癌的侵袭与转移,CCN3发挥作用机制的研究能为肝细胞肝癌的治疗提供新的思路。

Objective:To detect the expression and study the role of nephroblastoma overexpressed gene(NOV CCN3)in hepatocelluar carcinoma(HCC). Methods:The expressions of CCN3 m RNA in HCC samples and cell lines were detected by real-time PCR,and the expressions of CCN3 protein in HCC tissues and adjacent non-cancer tissues were assessed by Western blot. In addition,invasion of HCC cells was observed after overexpressing CCN3 by Transwell invasion assay and wound healing assay. Results:In the total of 50 paired HCC specimens,compared with the adjacent non-cancer tissues,the expression of CCN3 m RNA was up-regulated in 39 cases,and the expression of CCN3 protein in HCC tissues was higher than that in adjacent non-cancer tissues. Overexpression of CCN3 enhanced HCC-derived MHCC-97 H,SMMC-7721 cellular invasion in vitro. Conclusion:This study indicates CCN3 is frequently overexpressed in hepatocellular carcinoma,and contributes to tumor cell invasion. The study of CCN3 may provide a novel therapeutic idea for prevention and treatment of invasion in HCC.