HOXA5在原发性肝细胞肝癌发生发展中的作用

Effects of HOXA5 on carcinogenesis and progression of HCC

目的:探讨转录因子HOXA5在肝细胞性肝癌(hepatocellular carcinoma,HCC)发展中的作用。方法:利用qRT-PCR、蛋白质免疫印迹实验和免疫组化探究HOXA5在HCC组织和对应癌旁组织中的表达水平。构建敲低HOXA5的稳转人肝癌细胞系Hep3B、MHCC97H后应用平板克隆、CCK8、EdU检测细胞增殖能力;流式细胞术检测H2O2诱导的HCC细胞凋亡;Transwell实验检测细胞侵袭能力。蛋白质免疫印迹实验检测HOXA5敲低及过表达后对PI3K-AKT信号通路的影响。结果:HCC组织中的HOXA5表达量高于对应癌旁组织,敲低HOXA5后减弱HCC细胞增殖、侵袭以及对凋亡的抵抗能力,减弱PI3K-AKT信号通路的激活,而过表达HOXA5增强PI3K-AKT通路激活。结论:HCC组织中的HOXA5表达水平高于癌旁组织。HCC细胞中HOXA5表达水平改变可以影响其增殖、凋亡、侵袭能力,以及PI3K-AKT信号通路的状态。

Objective:This study aims to investigate the role of HOXA5 in HCC progression.Methods:RT-qPCR,Western blot,IHC were carried out to detect the expression of HOXA5 in HCC tissues and adjacent peritumor tissues.Hep3 B and MHCC97 H cells were chosen to establish the knockdown model.Colony formation assay,EdU assay,and CCK8 assay were performed to demonstrate the proliferation ability of HCC cells.Transwell assay and FACS technology were used to detected invasion and anti-apoptosis ability of HCC cells separately.Finally,Western blot was carried out to further confirm the possibile role that HOXA5 played in PI3 K-AKT signal pathway.Results:Expression of HOXA5 at mRNA and protein level in HCC tissues were significantly higher than that in adjacent peritumor tissues.Knockdown of HOXA5 could reduce the proliferation,invasion and anti-apoptosis ability of HCC cells.Knockdown of HOXA5 could decrease the expression of phosphorylated PI3 K and phosphorylated AKT,while ectopic expression of HOXA5 promoted the activation of this pathway.Conclusion:The expression of HOXA5 in HCC tissues is higher than that in adjacent tissues.Manipulation of HOXA5 expression in HCC cells had an impact on their proliferation,anti-apoptosis ability,invasion ability and activation of the PI3 K-AKT signaling pathway in vitro.