小鼠11β-羟类固醇脱氢酶敲除对肠道功能的影响

Effects of mouse 11β-hydroxysteroid dehydrogenase knockout on intestinal function

目的:探究小鼠11β-羟类固醇脱氢酶(11β-hydroxysteroid dehydrogenase,11β-HSD1)基因敲除对肠道功能的影响。方法:将C57/BL6为遗传背景的野生对照组及11β-HSD1基因敲除组各6只小鼠正常喂养至6~8周,观察小鼠及小肠组织大体变化,PCR及免疫组化确定其肠道功能及炎症变化。结果:11β-HSD1基因敲除能够显著改善肠道的炎症状态,减轻炎症,同时肠道黏膜通透性改变,肠道绒毛长度缩短,隐窝长度变长,杯状细胞数量增多,黏膜的屏障功能增强。结论:本研究成功建立了小鼠11β-HSD1基因敲除模型,证实了肠道在11β-HSD1基因敲除后炎症状态改善,为进一步11β-HSD1肠道组织特异性敲除提供了研究基础。

Objective:This study aims to observe the changes of intestinal function in 11β-hydroxysteroid dehydrogenase(11β-HSD1)knockout mice.Methods:Six 11β-HSD1 knockout mice were paired with 6 control mice with C57/BL6 genetic background both fed standard chow ad libitum for a period of 6-8 weeks.The changes in mice characteristics and intestinal morphometry were observed.The intestinal function and inflammatory alterations were determined by PCR and immunohistochemistry.Results:11β-HSD1 gene abortion significantly decreased the intestinal inflammation.Intestinal mucosal permeability was altered,intestinal villus length was shortened,crypt depth became deeper,goblet cell number increased,and mucosal barrier function was enhanced.Conclusion:This study successfully established a 11β-HSD1 knockout mice model,which showed inflammation amelioration in intestinal tract after 11β-HSD1 gene knockout,providing a research foundation for further specific knockouting 11β-HSD1 in intestinal tissue.