岩藻多糖对炎症性肠病的作用及其机制

The effects and mechanism of fucoidan on inflammatory bowel disease

目的:探讨岩藻多糖抑制炎症性肠病(inflammatory bowel disease,IBD)的作用及其机制。方法:将小鼠随机分为实验组和对照组(每组5只),实验组给予岩藻多糖灌胃2周,对照组给予饮用水灌胃2周,随后两组小鼠均自由饮用溶有葡聚糖硫酸钠(dextran sulfate sodium salt,DSS)的水溶液,构建IBD小鼠模型。检测小鼠体重、脾脏重量、结肠长度及重量,HE染色观察结肠组织病理变化,观察大便性状并评分;运用实时荧光定量PCR(real-time fluorescence quantitative PCR,qPCR)检测炎症因子、趋化因子和紧密连接蛋白的mRNA水平变化。结果:岩藻多糖处理的实验组小鼠肠炎相关指标显著优于对照组,炎症程度减弱。结论:岩藻多糖能够预防和抑制IBD的发生,为预防和治疗IBD提供新方法和依据。

Objective:This study aims to investigate the effect and mechanism of fucoidan inhibiting inflammatory bowel disease(IBD).Methods:Mice were randomly divided into experimental group and control group(n=5,each group).The experimental group was intragastrically with fucoidan,meanwhile,the control group was given drinking water.Two weeks later,both groups drank dextran sulfate sodium salt(DSS)to make mouse model of IBD.Body weight,spleen weight,colon length and weight of mouse were measured.Pathological changes of colon tissue was observed by HE staining,stool property was observed and scored,and mRNAs of inflammatory factors,chemokines and tight junction proteins were detected by real-time fluorescence quantitative PCR.Results:Intestinal inflammation related indexes were better in experimental group treated with fucoidan than in the control group,and the degree of inflammation was reduced.Conclusion:Fucoidan can prevent and inhibit the occurrence of IBD.This study will provide a new method and evidence for the prevention and treatment of IBD.