基于HPLC结合化学计量学的壮瑶药小槐花指纹图谱研究

Study on Fingerprint of Desmodium Caudatum Based on HPLC and Chemometrics

目的建立基于HPLC结合化学计量学的壮瑶药小槐花指纹图谱,为其质量控制提供科学的评价方法。方法采用HPLC法,色谱柱为ZORBAX SB-C18(5μm,4.6mm×250mm),检测波长为330nm,以甲醇-0.2%磷酸水溶液梯度洗脱,流速为1.0mL/min,通过中药色谱指纹图谱相似度评价结合聚类分析和主成分分析对10批次小槐花样品进行研究。结果建立了小槐花HPLC指纹图谱,标定出13个共有峰,指纹图谱相似度均在0.9以上,聚类分析将小槐花样品大致分为4类,与主成分分析结果基本一致,影响药材质量差异是多成分协同作用的结果。结论将小槐花指纹图谱采用HPLC结合化学计量学进行分析,方法专属性强,精密度、稳定性及重现性良好,可作为小槐花质量评价和控制的有效方法。

OBJECTIVE To establish a fingerprint of Desmodium caudatumbased on HPLC and chemometrics,and to provide a scientific evaluation method for its quality control.METHODS A HPLC method was developed for the determination of10 batches of Desmodium caudatum by using ZORBAX SB-C18(5μm,4.6 mm×250 mm)column with a detection wavelength of 330 nm and gradient elution with methanol-0.2% phosphoric acid solution at a flow rate of 1.0 mL/min.RESULTS The HPLC fingerprint of Desmodium caudatum was established,13 common peaks were calibrated,and the similarity of fingerprint was above 0.9.Cluster analysis classified the samples into 4 categories,which was basically consistent with the results of principal component analysis.The effect of the quality difference of Desmodium caudatum was the result of the synergistic action of multi-components.CONCLUSION The fingerprint of Desmodium caudatum was analyzed by HPLC combined with chemometrics.The method has strong specificity,good precision,stability and reproducibility.It can be used as an effective method for quality evaluation and control of Desmodium caudatum.