少弱精子症患者精子蛋白质组学的生物信息学研究

Bioinformatics study of sperm proteomics in oligoasthenozoospermia

目的:采用TMT技术筛选少弱精子症患者精子差异蛋白,并通过生物信息学分析,旨在为少弱精子症的研究提供研究方向和理论依据。方法:收集并随机取30例少弱精子症患者和30例正常男性的精液标本,运用TMT技术蛋白质组学分析筛选出少弱精子症患者精子差异表达的蛋白质,并进行基因本体(GO)分析和KEGG信号通路生物信息学分析。结果:通过蛋白质组学技术获得差异蛋白1 199种,其中上调蛋白663种,下调蛋白536种。GO分析结果初步提示差异蛋白主要聚集在核糖体成分、核糖体功能方面。KEGG信号通路分析发现差异蛋白涉及244条信号通路。结论:少弱精子症患者精子差异蛋白涉及复杂的生物过程、分子功能和信号通路,并且蛋白质组学筛查与其生物信息学分析有助于少弱精子症发病机制的研究。

Objective:To identify differentially expressed proteins in the sperm of oligoasthenozoospermia(OAZ)patients and provide some theoretical evidence for the study of OAZ.Methods:We collected semen samples from 30 OAZ patients and another 30 normal healthy males.Using the tandem mass tag(TMT)and proteomic technology,we identified differentially expressed proteins in the sperm of the OAZ patients and normal subjects,followed by gene ontology(GO)analysis and bioinformatics analysis of the Kyoto encyclopedia of genes and genomes(KEGG)signaling pathways.Results:A total of 1 199 differentially expressed sperm proteins were obtained from the semen samples of the subjects by proteomic technology,of which 663 were up-regulated and 536 down-regulated.GO analysis preliminarily indicated that the differential proteins played a leading role in the composition and function of the ribosome,while KEGG pathway analysis showed that the differential proteins were involved in 244 signaling pathways.Conclusion:Differentially expressed proteins in the sperm of OAZ patients involve complex biological processes,molecular functions and signaling pathways,and proteomic screening and bioinformatics analysis are helpful for the study of the pathogenesis of oligoasthenozoospermia.