摘要
对已经完成全序列测定的 12个SARS病毒基因组进行了多序列比对 ,发现序列主体部分 2 970 8b具有99 82 %的相同碱基 ,除 2个序列各有 5个和 6个碱基的缺失外 ,其余部分共有 4 2个位点核苷酸碱基的差异 ,其中2 8个位点的碱基差异可引起氨基酸残基改变。利用蛋白质二级结构和跨膜螺旋预测以及蛋白质定位等生物信息学工具 ,分析了这些产生氨基酸改变部位的蛋白质构像 ,推测了可能产生的结构和功能改变 ,为进一步生物学实验提供参考。所有分析结果同时在北京大学生物信息中心抗SARS网站 (antisars.cbi.pku .edu .cn)上发布。
Multiple sequence alignment among 12 complete SARS coronavirus (SARS CoV) sequences reveals that the major parts of 29708 b of the genomes have 99 82% identical bases.Forty two nucleotide mismatches were found in addition to the five and six gaps in two genomes.Among them,28 mismatches result in changes of amino acid in the encoded proteins.Analysis of the changes implies possible effect on the Spike and Membrane protein of the virus,while most of the other changes seem not very significant to alter the structure and function of the proteins.These results have been released on the anti sars web site maintained by the Centre of Bioinformatics,Peking University (antisars.cbi.pku.edu.cn) and may be of help for further experimental study.
基金
863基金(2001AA231011,2002AA231061)
国家自然科学基金(30170232)资助
