摘要
用经筛选的 8个随机引物对稻蝗属 (OxyaAudient Serville)中的中华稻蝗 (Oxyachinensis)、日本稻蝗 (Oxyajaponica)、山稻蝗 (Oxyaagavisa)、短翅稻蝗 (Oxyabrachyptera)和小稻蝗 (Oxyaintricata)等 5个种 4 1个个体进行扩增 ,均扩增出特异性产物 ,在 96条清晰稳定的扩增片段中 ,多态性片段为 95条 ,种间共有片段 1条 (引物S36 2扩增的约 85 0bp的片段 ) ,所获片段的长度大小为 2 0 0～ 2 5 0 0bp ,单个引物扩增的条带数在 8～ 16之间 ,平均为 12。根据扩增结果 ,应用SPSS软件包中的Between groupslinkage法进行聚类分析 ,得到 5种稻蝗的分子系统树。结果显示 :中华稻蝗与短翅稻蝗关系密切 ,日本稻蝗与山稻蝗关系较近 ,而小稻蝗与它们的亲缘关系较远。聚类图所呈现的种间关系与传统的形态及细胞分类学结果基本一致 ,5种稻蝗亲缘关系的远近程度得以清楚显示 。
The molecular phylogenetic relationships of five species of Oxya Audient serville including O.chinensis,O.brachyptera,O.agavisa,O.japonica and O.intricata were studied with RAPD.Genomic DNA of forty one individuals were amplified with eight oligonucleotide (10 mer) primers which were previously selected,the specifical bands occured in all them,a total of 96 clear and reproducible bands (rang from 200～2500 bp) were generated,95 of which were polymorphic band,the only one band (850 bp) which was amplified with S362 was common to five species of Oxya .The obtaining segments of individual primer were between 8～16,the average was 12.A molecular phylogenetic tree based on was constructed Euclidean distance among five species of rice grasshopper by between groups linkage method,the result indicated O.chinensis was closely related to O.brachyptera ,the genetic relationship of O.japonica and O.agavisa was also close,whereas O.intricata had far phylogenetic relationship with them.The results of dendrogram were consistent with the previous conclusion of morphologic classification and cytotaxonomy,and suggested RAPD was suitable for analysis of phylogenetic relationships among species of Oxya .
基金
国家自然科学基金资助项目(编号 :3 0 0 70 112)
山西省自然科学基金资助项目(编号 :9910 96)资助~~
