新生儿CYP2E1基因5′端RsaⅠ位点多态性和PON2基因148位点多态性与早产的关系

Associations of RsaⅠpolymorphism at the 5′ flanking region of CYP2E1 and PON2 148 Polymorphism in Neonates with Preterm Delivery

为探讨新生儿细胞色素P4 5 0 2E1(CYP2E1)基因多态性和对氧磷酶 2 (二乙基对硝基苯磷酸酯酶 2 )基因(PON2 ) 14 8位点多态性对早产的影响 ,采用横断面调查方法 ,使用统一的调查表 ,由安庆市各县医院对入院分娩孕妇及其单胎、活产、早产和对照新生儿进行调查 ,共得到有效样本 2 0 9个母亲 新生儿对。单因素分析结果显示 :CYP2E1野生纯合子基因型 ( + + )与突变纯合子基因型 ( - - ) 杂合子基因型 ( + - )比较 ,对早产的影响不具有统计学意义。而PON2Ala14 8Ala纯合子基因型与Gly14 8Gly纯合子基因型 Ala14 8Gly杂合子基因型比较 ,对早产的影响具有显著的统计学意义。进一步分析CYP2E1基因 5′端RsaⅠ位点多态性和PON2基因 14 8位点多态性是否存在交互作用 ,结果显示 :CYP2E1野生纯合子基因型和PON2Ala14 8Ala纯合子基因型这一组合与参照组比较 ,对早产的影响有显著的统计学意义。基因CYP2E15′端RsaⅠ位点多态性与新生儿早产不相关 ,但基因PON2 14 8位点多态性与新生儿早产相关 ,且CYP2E15′端RsaⅠ位点多态性和PON2 14

The objective is to investigate whether Rsa Ⅰpolymorphism at the 5′ flanking region of cytochrome P450 2E1 gene ( CYP2E1 ) and paraoxonase 2 gene polymorphism ( PON2 148) in neonates are associated with preterm delivery.Using standard questionnaires,209 singleton live born mother neonate pairs (include preterm cases and term controls) were investigated by the trained field workers with cross sectional survey at the hospitals in Anqing,Anhui Province,China. Epidemiological and clinical data and blood samples were obtained from 209 mother neonate pairs. CYP2E1 homozygous wild type (+/+) is not associated with a shortened gestation among neonates,compared with CYP2E1 homozygous mutant type (-/-)/ CYP2E1 heterozygote (+/-) before and after adjustment confounders,however, PON2 Ala148Ala homozygote is significantly associated with a shortened gestation among neonates.When Rsa Ⅰpolymorphism at the 5′ flanking region of CYP2E1 and PON2 148 polymorphism were considered jointly, a significant shortened gestation was observed among neonates with the combined genotype of CYP2E1 homozygous wild type and PON2 Ala148Ala homozygote.In conclusion, Rsa Ⅰpolymorphism at the 5′ flanking region of CYP2E1 in neonates is not associated with preterm delivery,however, PON2 148 polymorphism in neonates is significantly associated with preterm delivery.Furthermore,the gene interaction between Rsa Ⅰpolymorphism at the 5′ flanking region of CYP2E1 and PON2 148 polymorphism in neonates is significantly associated with preterm delivery.