借助耐盐菌以餐厨垃圾为碳源合成PHA条件优化研究

Optimization of PHA conditions for synthesis of food waste by using salt-tolerant bacteria

目的:分析耐盐菌以餐厨垃圾为碳源合成PHA的条件优化影响。方法:从含盐量高的生活垃圾渗沥液处理系统和盐碱地中筛选分离出PHA合成能力较强的菌株,经过16SrDNA分析确定其菌种,然后在不同条件(接种率、pH值、餐厨废油添加量)下进行PHA含量的测定。对比分析条件的不同对于PHA合成量的影响。结果:本次分离出的三种菌株分别为苏云金芽孢杆菌菌株IARI-UPS6,苏云金芽孢杆菌菌株L14,蜡样芽胞杆菌菌株FORC_024;菌株接种率和餐厨废油添加量不会影响耐药菌的PHA合成,而pH值对于耐盐菌PHA的合成有较大影响,而且不同菌种有各自最适合的合成pH值。结论:对接种量和pH值、餐厨废油添加量三种因素对PHA合成量的影响进行了优化,发现培养的pH值对于PHA合成量有最明显的影响,因此未来可以思考从此入手,优化PHA合成环境,提高PHA合成量。

Objective: To analyze the optimal effects of salt-tolerant bacteria on the synthesis of PHA by using kitchen waste as carbon source. Methods: The strains with strong PHA synthesis ability were screened out from the domestic garbage leachate treatment system and saline-alkali soil. The strains were determined by 16 S r DNA analysis, and then under different conditions(vaccination rate, pH value, The PHA content was measured under the amount of kitchen waste oil added. The effect of the difference in analytical conditions on the amount of PHA synthesis was compared. Results: The three strains isolated were Bacillus thuringiensis strain IARI-UPS 6,Bacillus thuringiensis strain L14, Bacillus cereus strain FORC024;the inoculation rate and the amount of waste oil added to the kitchen did not affect the drug-resistant bacteria. The PHA synthesis, while the pH value has a great influence on the synthesis of salt-tolerant PHA, and different strains have their most suitable synthetic pH values. Conclusion: The effects of inoculum size, pH value and amount of kitchen waste oil on the amount of PHA synthesis were optimized. It was found that the pH value of culture had the most obvious influence on the amount of PHA synthesis. Therefore, we can think about it in the future. Optimize the PHA synthesis environment and increase the amount of PHA synthesis.