摘要
为了解吉林山鸡种群的遗传多样性,采用PCR技术及测序技术对20只吉林山鸡的mtDNA Cytb基因序列进行了分析。结果表明,吉林山鸡mtDNA Cytb基因序列中,共检测到核苷酸多态位点11个,20个个体具有3种单倍型;吉林山鸡与美国山鸡亲缘关系较近,与北京山鸡和甘肃山鸡亲缘关系较远。
In order to understand the genetic diversity analysis of Phasianus colchicus in Jilin Province, the PCR assay and sequencing technology were employed to analyze the mitochondrial cytochrome b gene(mtDNA Cytb) of 20 Phasianus colchicus from Jilin Province. The results showed that a total of 11 nucleotide polymorphism sites were found and 3 haplotypes were identified in the analyzed mtDNA Cytb sequences. The phylogenetic analysis result indicated that Phasianus colchicus in Jilin Province was genetically close to Phasianus colchicus in USA, and was genetically distant to that in Beijing and Ganshu Province.
出处
《畜牧与饲料科学》
2015年第9期7-8,共2页
Animal Husbandry and Feed Science
基金
吉林农业科技学院青年基金项目(吉农院合字[2014]第228号)
吉林农业科技学院博士启动基金项目(吉农院合字[2012]第304号)
关键词
吉林山鸡
CYTB基因
遗传多样性
系统进化分析
Phasianus colchicus in Jilin Province
cytochrome b gene
genetic diversity
phylogenetic analysis
