IL-10对人肾小球系膜细胞细胞周期负调控蛋白p27的调节

Effects of IL-10 on the Cell Cycle Negative Regulatory Protein p27 in Human Glomerular Mesangial Cells

观察白细胞介素 10 (IL 10 )对体外培养的人肾小球系膜细胞 (HMC )增殖影响 ,并探讨IL 10对HMC细胞周期负调控蛋白p2 7的影响 ,旨在了解其调节HMC增殖的内在机制。HMC用含 5 %FCS的RPMI16 4 0培养 ,加入IL 10刺激后 ,应用流式细胞术分别测定HMC细胞增殖周期的变化以及p2 7的水平。结果表明IL 10可抑制血清诱导的HMC增殖 ,IL 10显著减少了HMCG0 /G1期细胞进入S期和G2 /M期 ;2 5ng/mlIL 10明显地上调细胞周期负调控蛋白p2 7水平 ,是血清刺激组的 1 7倍 (P <0 0 5 )。IL 10抑制血清诱导的HMC增殖的作用机制可能部分是通过上调细胞周期负调控蛋白p2 7来实现的 ,提示IL

To observe the effects of IL 10 on the proliferation of human glomerular mesangial cells and the cell cycle negative regulatory protein p27,the glomerular mesangial cells were cultivated in RPMI 1640 supplemented with 5% FCS in the presence or absence of 25 ng/ml of IL 10 Flow cytometry was used to analyze the proliferation of mesangial cells and to determine the level of p27 It was found that IL 10 could inhibit the proliferation of these cells in vitro and remarkably reduce the numbers of mesangial cells from G0/G1 phase of cell cycle entering into S and G2/M phase,thus suppressing the proliferation of mesangial cells After treatment wih IL 10,the level of p27 was up regulated to 1 7 times It concludes that the effects of IL 10 to inhibit proliferation of mesangial cells may be mainly through the regulation of the cell cycle protein p27 and IL 10 may play an important role in the pathogenesis of prolifrative glomerulonephritis