肝癌患者CIK细胞的诱导及对肝癌细胞毒作用的研究

Study on the Induction of Cytokine-Induced Killer and the Cytotoxic Effect of These Cells on Hepatocellular Cells

观察肝癌患者PBMC在体外诱导成CIK细胞的能力及其对肝癌细胞的细胞毒作用。对比正常人组和肝癌患者组CIK细胞和LAK细胞之间的扩增差异。用流式细胞仪检测CIK细胞表面标志CD3、CD5 6 ,3 H TdR释放法检测CIK细胞、LAK细胞对肝癌细胞系SMMC 772 1等多种细胞系的细胞毒作用。结果显示 ,肝癌组和正常组的CIK细胞扩增倍数分别达 6 4 3倍和6 7 4倍 ,CD3+CD5 6 +细胞扩增倍数均达 6 0 0倍以上 ,在细胞扩增曲线及细胞表面标志上无差异 ,远大于LAK细胞 ;肝癌组CIK对肝癌细胞SMMC 772 1、Bel 74 0 2、Hep 3b杀伤能力均达 6 5 %～ 81% ,与正常组相同 ,且与对肠癌细胞系HIC 2 5 1杀伤能力无差异 ;对正常胎肝细胞系L 0 2的细胞毒作用 <5 % ;肝癌患者CIK对耐药的和未诱导耐药的K5 6 2细胞细胞毒作用均达到 70 %左右。肝癌患者CIK和正常人CIK一样对肝癌细胞有很强的细胞毒作用 ,对耐药肿瘤同样有效 ,对正常肝细胞无损伤 。

To observe the induction of cytokine induced killer (CIK) in PBMC of patients with liver cell carcinoma and the cytotoxic effect of these cells on the hepatocellular cells (HCC),the differences of amplification of CIK and LAK in patients with liver cell carcinoma and normal adult controls were determined by testing the cell surface markers of CIK,CD3 and CD56 The cytotoxic effects of CIK and LAK to target cells (SMMC 7721) were demonstrated by 3 H TdR release method The results showed that the amplifications of cell growth increased vigorously up to more than 60 times both in HCC group and normal control group,and those of CD3 + and CD56 + cells elevated up to more than 600 times As to the cytotoxicity rate of the expreimental group,it measured as 65%～81%,just as the same as those of the control group