常夏石竹的组织培养与快速繁殖

Tissue Culture and Rapid Propagation of Dianthus Plumarius

用常夏石竹的茎尖作为外植体进行培养,成功地建立了常夏石竹的快速繁殖程序。常夏石的茎尖接种在MS+6-BA2.0mg/L+NAA0.1mg/L的培养基上能够形成绿色愈伤组织;把带芽点的愈伤组织转移至培养基:MS+6-BA1.5mg/L+NAA0.1mgL上继代效果较好;不定芽在MS+IBA0.3mg/L+0.5％活性碳的培养基上易生根。

Used as an explant, the stem tip of Dianthus plumarius is cultured, and the rapid propagation procedure of Dianthus plumarirs is built successfully. The stem tip of Dianthus plumarius is inoculated in the medium with the formula of MS+6-BA2. 0mg/L+NAA0. 1mg/L,as a result the green callus can form; When the callus with buds is removed to the medium with the formula of MS+6-BA1.5rag/L+ NAA0.1mg/L, the following generation can achieve better results; The adventious bud takes root easily on the active carbon medium with the formula of MS+IBA0.3rag/L+0.50%.