草坪种子品种快速鉴定技术研究 II几种冷季型草坪草过氧化物同工酶谱计算机分析

Studies on rapid identification techniques for turfgrass seed varieties. II Computer analysis of peroxidase isozyme bands of cold-season turfgrasses

采用等电聚焦电泳技术对适于西北地区的几种冷季型草坪草种进行了品种真实性鉴定研究,并使用国际认证的计算机软件(ImageMaster1D)进行同工酶谱带分析,对检测结果进行了定性定量评定,充分表明了品种间基因多态性,证明此方法是草坪草品种鉴别的有效测定技术。

In this paper, the isoelectric focusing electrophoresis technology was utilized to identify the authenticities of several coldseason turf grasses in northwestern China, the internationally admitted computer analysis software (ImageMaster 1D)was used to analyze peroxidase isozyme bands of the turfgrassesThe qualitative and quantitative evaluation of test resuls indicated the gene polymorphism among different cultivars, proving that the isoelectric focusing electrophoresis technology is an effective measure in turfgrasses cultivar identification