摘要
Aim To develop a rapid, effective method for the determination of flavonoidsand ginsenosides in one injection and evaluate the flavonoids and ginsenosides content to controlthe ratio of Epimedium and Ginseng herbs in botanical combinations. Methods The quality evaluationwas determinatted using reversed-phase high performance liquid chromatog-raphy (HPLC), referred bythe major flavonoids from Epimedium, epimedin A, epimedin B, epimedin C, and icariin as thestandards, and the major ginsenosides Rg_1, Re, Rf, Rb_1, Rb_2, and Rd as the standards, includedEpimedium brevicornum Maxim., E. sagittatum (Sieb. et Zucc) Maxim., E. koreanum NaKai, P. ginseng C.A. Meyer, P. quinquefolium L., P. notoginseng and some products containing the above herbs. ResultsThe main flavonoids and ginsenosides could be clearly resolved in the single analysis. ConclusionThe results can be effectively used in evaluating qualitatively and quantitatively the ration ofEpimedium and Ginseng contained products.
Aim:To develop a rapid,effective method for the detemination of flavonoids and ginsenosides in one injection and evaluate the flavonoids and ginsenosides content to control the ratio of Epimedium and Ginseng herbs in botanical combinations.Methods: The quality evaluation was determinatted using reversed-phase high performance liquid chromatog raphy(HPLC),referred by the major flovonoids from Epimedium,epimedin A,epimedin B,epimedin C,and icariin as the standards,and the major ginsenosides Rg1,Re,Rf,Rb1,Rb2,and Rd as the standards,included Epimedium brevicornum Maxim.,E.sagittatum(Sieb.et Zucc)Maxim.,E.koreanum NaKai,P.ginseng C.A.Meyer,P.quinquefolium L.,P.notoginseng and some products containing the above herbs.Results:The main flavonoids and ginsenosides could be clearly resolved in the single analysis.Conclusion.The results can be effectively used in evaluating qualitatively and quantitatively the ration of Epimdium and Ginseng contained products.
