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Calcium Glucarate Prevents Tumor Formation in Mouse Skin 被引量:3

Calcium Glucarate Prevents Tumor Formation in Mouse Skin
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摘要 Objective Calcium Glucarate (Cag), Ca salt of D-glucaric acid is a naturally occurring non-toxic compound present in fruits, vegetables and seeds of some plants, and suppress tumor growth in different models. Due to lack of knowledge about its mode of action its uses are limited in cancer chemotherapy thus the objective of the study was to study the mechanism of action of Cag on mouse skin tumorigenesis. Methods We have estimated effect of Cag on DMBA induced mouse skin tumor development following complete carcinogenesis protocol. We measured, epidermal transglutaminase activity (TG), a marker of cell differentiation after DMBA and/or Cag treatment and [3H] thymidine incorporation into DNA as a marker for cell proliferation. Results Topical application of Cag suppressed the DMBA induced mouse skin tumor development. Topical application of Cag significantly modifies the critical events of proliferation and differentiation TG activity was found to be reduced after DMBA treatment. Reduction of the TG activ Objective Calcium Glucarate (Cag), Ca salt of D-glucaric acid is a naturally occurring non-toxic compound present in fruits, vegetables and seeds of some plants, and suppress tumor growth in different models. Due to lack of knowledge about its mode of action its uses are limited in cancer chemotherapy thus the objective of the study was to study the mechanism of action of Cag on mouse skin tumorigenesis. Methods We have estimated effect of Cag on DMBA induced mouse skin tumor development following complete carcinogenesis protocol. We measured, epidermal transglutaminase activity (TG), a marker of cell differentiation after DMBA and/or Cag treatment and [3H] thymidine incorporation into DNA as a marker for cell proliferation. Results Topical application of Cag suppressed the DMBA induced mouse skin tumor development. Topical application of Cag significantly modifies the critical events of proliferation and differentiation TG activity was found to be reduced after DMBA treatment. Reduction of the TG activity was dependent on the dose of DMBA and duration of DMBA exposure. Topical application of Cag significantly alleviated DMBA induced inhibition of TG. DMBA also caused stimulation of DNA synthesis in epidermis, which was inhibited by Cag. Conclusion Cag inhibits DMBA induced mouse skin tumor development. Since stimulation of DNA synthesis reflects proliferation and induction of TG represents differentiation, the antitumorigenic effect of Cag is considered to be possibly due to stimulation of differentiation and suppression of proliferation.
出处 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2003年第1期9-16,共8页 生物医学与环境科学(英文版)
基金 Financial support was provided by Indian Council of Medical Research,New Delhi.
关键词 CAG Skin tumor CHEMOPREVENTION TG DNA synthesis SKIN TRANSGLUTAMINASE Cag Skin tumor Chemoprevention TG DNA synthesis Skin Transglutaminase
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  • 1郭元.中国能源的需求增长及对能源供应的挑战[J].化工技术经济,2005,23(7):8-9. 被引量:1
  • 2Okada D R, Johnson G, Liu Z L, et al. Early detection of infarct in reperfused canine myocardium using Tc-99m-glucarate[J]. Journal of Nuclear Medicine, 2004, 45:655-664.
  • 3Khaw B A, Da Silva J, Petrov A, et al. Indium 111 antimyosin and Tc-99m glucaric acid for noninvasive identification of oncotic and apoptotic myocardial necrosis[J]. Journal of Nuclear Cardiology, 2002, 9: 471-481.
  • 4Mariani G, Villa G, Rossettin P F, et al. Detection of acute myocardial infarction by Tc-99m-labeled D-glucaric acid imaging in patients with acute chest pain[J]. Journal of Nuclear Medicine, 1999, 40: 1832-1839.
  • 5Arteaga de Murphy C, Ferro-Flores G, Villanueva-Sanchez O, et al. 99m Tc-glucarate for detection of isoproterenol-induced myocardial infarctioninrats[J].InternationalJournalofPharmaceutics, 2002, 233: 29-34.
  • 6Liu Z, Stevenson G D, Kastis G A, et al. 99mTc-glucarate: Detecting human breast tumor in SCID mice with high resolution Fastspect imaging[J]. Journal of Nuclear Medicine, 2002, 43 (Suppl) : S273.
  • 7Gambini J P, Nunez M, Cabral P, et al. Evaluation of patients with head and neck cancer by means of 99mTc-glucarate[J]. Journal of Nuclear Medicine Technology, 2009, 37: 229-232.
  • 8Viswanathan A, Kiely D E. Mechanisms for the formation of diamides and Polyamides by aminolysis of D-glucaric acid esters[J]. Journal of Carbohydrate Chemistry, 2003, 22: 903-918.
  • 9Chen L, Kiely D E. Synthesis of stereoregular head, tail hydroxylated nylons derived from d-glucose[J]. Journal of Organic Chemistry 1996, 61:5847-5851.
  • 10Styron S D, Kiely D E, Ponder G. Alternating stereoregular Head, tail-tail, Head-poly (alkylene D-glucaramides ) derived from a homologous series of symmetrical diamido-Di-D-glucaric acid monomers[J]. Journal of Carbohydrate Chemistry, 2003, 22: 123-142.

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