玉米抗甘蔗花叶病毒资源的遗传多样性研究(英文)

Genetic Diversity of Elite Maize Germplasm for Resistant to SCMV

利用人工接毒方法对 4 6份我国主要玉米自交系进行了两年抗甘蔗花叶病毒鉴定 ,筛选出高抗系 8份 (K2 2、CN96 2、P138、齐 318、中自 0 1、金黄 96 B、齐 319、Pa4 0 5 ) ,抗病系 7份 (旱 2 1、中自 0 3、旱 2 3、农大 178、获白、K12、黄早四 )。用 SSR标记研究了 4 6份自交系的遗传多样性。 4 9对引物共检测出 16 8个等位基因变异 ,每对引物检测等位基因2～ 10个 ,平均为 3.4 3个。 U PGMA聚类分析表明 ,供试自交系可分为 7群 ,划群结果与系谱和育种家经验基本相符。 15份抗病毒自交系分散于 4群 (A、B、E、G) ,依据杂种优势原理 ,可用于改良同一群内感病系的抗性 ;其中群 E和亚群 GII被鉴定为抗病毒种质 ,可用于组建抗病群体。本文的研究结果为抗甘蔗花叶病毒玉米种质改良提供了重要信息。

Forty six elite maize( Zea mays L.) inbred lines were evaluated for reaction to sugarcane mosaic virus under field conditions with artificial inoculation in 1999 and 2000. The results showed that 8 lines (K22, CN962, P138, Qi318, Zhongzi01, Jinhuang96B, Qi319, and Pa405)were highly resistant to SCMV,and 7(Han21, Zhongzi03, Han23, Nongda178, Huobai, K12,and Huangzao4)were resistant. The germplasm basis for resistance to SCMV in 46 inbreds was investigated by SSR markers. The average number of alleles per SSR locus was 3.43 with a range from 2 to 10. The UPGMA cluster analysis showed that the 46 inbreds could be classified into 7 distinct groups with several subgroups, which were generally consistent to their known pedigree information and breeder's experiences. Fifteen resistant inbreds were found in 4 groups (A,B,E,G), which could be used in germplasm improvement for SCMV resistance based on heterotic pattern, respectively. Group E and subgroup GII were identified as two important sources of resistance, which can be employed to synthesize the composites for SCMV resistance. The study provided useful information for germplasm improvement for resistance to SCMV.