中国人甲状腺乳头状癌中ret基因重排及类型的分析研究

Analysis of ret gene rearrangements and forms in papillary thyroid carcinomas (PTC) of a Chinese population

目的 建立ret基因重排的分析方法 ,提示中国人甲状腺乳头状癌中是否存在原癌基因ret重排及重排类型。方法 应用RT PCR方法对 65例甲状腺乳头状癌石蜡切片标本进行ret基因重排检测 ,并对扩增结果进行测序鉴定。结果 在提取RNA成功的 38例甲状腺乳头状癌 (PTC)标本中 ,有 71 %发生ret重排 ,其中 1 0 5 %表达PTC1 ;5 3 %表达PTC3 ;5 3 %表达PTC4 ,以PTC1发生的频率较高。仅 1例表达PTC2与PTC3 PTC4混合型重排 ,中国人PTC可能与ret和H4及ELE1重排激活相关。结论 中国人PTC组织中存在 4种重排 ,且以PTC1较为常见。单一标本中有多种重排形式并存 ,PTC1 +3重排形式 1 0例、3例PTC1 +4、1例PTC2 +3 +4、1例PTC3 +4、4例PTC1 +3 +4,单一标本中多种重排形式并存的比例高达 50 % ,但与癌症的恶性程度。

Objective\ To establish a method of detecting ret gene arrangements and to investigate the rearrangements and forms of ret oncogene in papillary thyroid carcinomas (PTC) of a Chinese population. Methods\ RT\|PCR was performed to detect the ret arrangements of paraffin\|embeded specimens of 65 patients with PTC and the amplified products were cloned into T vector and sequenced using specific primers for ret/PTC 1,2,3 and 4. Result\ RNA was successfully extracted from 38/65 cases,of which 71% were found expressing ret/PTC,including 10\^5% PTC1,5\^3% PTC3 and 5\^3% PTC4.The frequency of PTC1 was significantly higher than that of PTC2, which was found only in one patient.It was indicated that PTC in the Chinese population was related with ret rearrangements with H4 and ELE1.Previous studies also suggested that PTC1 and PTC3 occurred frequently in PTC patients. Conclusions\ PTC1,2,3 and 4 forms are present in Chinese PTC patients,and PTC1 is more frequently found ,consistenting with other reports.Furthermore, multiple rearrangements could be present in a single specimen,with a frequency of 50% for all the detected cases.The present data suggest that the multiple rearrangements in a single specimen may be not linked to the degree of malignancy and metastasis.\;