甘薯体细胞胚状体及其在脱毒、扩繁中的应用

Somatic Embryogenesis of Sweetpotato and Its Application in Virus-free and Propagation

通过甘薯徐薯 18茎尖体细胞培养诱导胚性愈伤组织和胚状体 ,建立了体细胞无性系。胚性愈伤和胚状体诱导培养基为 MS附加 0 .5～ 2 .0 m g/ L 2 ,4 - D、0 .5 mg/ L NAA、0 .1mg/ L BA及 10 0 mg/ L HL。研究结果表明 :采用茎尖体细胞胚胎发生途径能够同时实现试管苗的深度脱毒及大幅度提高扩繁量 ,脱病毒率达 95 % ,扩繁量达 382 .9倍。田间种植该脱病毒种苗能使甘薯产量比茎尖培养试管苗提高 5 0 %～ 10 0 %。

Embryogenic callus and somatic embryogenesis were induced from the apical meristem of sweetpotato cultival Xushu 18( Ipomoea batatas (L.) Lam.) in MS medium supplemented with 0 5—2 0 mg/L 2,4 D, 0 5mg/L NAA, 0 1mg/L BA and 100mg/L HL. The results showed that the multiplication rate of plantlets from the embryogenesis could reach 382 9 times and the virus free rate in the plantlets was about 95%. The field experiment indicated that the yield of sweetpotato from embryogenesis plantlets were increased 50%—100% than that directly from shoottip culture.