基质细胞支持的体外扩增造血细胞对致死剂量照射小鼠造血恢复的促进作用

Effects of expanded bone marrow cells supported by stromal cells on hematopoietic repair in lethally irradiated mice

目的 研究基质细胞支持条件下体外扩增的造血细胞回输体内后 ,促进经致死剂量照射小鼠造血功能恢复的作用。方法 在培养的小鼠骨髓基质细胞层存在的条件下 ,加入或不加入几种细胞因子组合 ,分别在小鼠骨髓单个核细胞液体培养体系中进行造血细胞体外扩增。将不同条件下扩增的细胞经尾静脉回输至经致死剂量照射的小鼠体内 ,并与单纯细胞因子存在条件下的扩增及未扩增造血细胞进行比较 ,动态观察小鼠的外周血象变化及其一般状况和生存率。结果①单纯细胞因子介导小鼠骨髓单个核细胞 (BMMNC)体外扩增后并不能增进这些细胞促进造血恢复的能力 ;②含有骨髓基质细胞底层的体外扩增 ,无论是否加入细胞因子 。

Objective\ To compare the effects of bone marrow cells expanded under different conditions on hematopoietic repair of radiation injuy. Methods\ In the liquid expanded cultural system with several cytokines and/or a bone marrow stromal cell layer,bone marrow mononuclear cells of mice were expanded for 5 days.Then,the expanded cells were transplanted to lethally irradiated mice via the caudal vein.The hematopoietic recovery of mice after transplantation was assessed by analysing the peripheral blood Hb,WBC,TBC and obser\|ving the survival states. Results\ Ex vivo expansion of bone marrow mononuclear cells with combined use of cytokines under our cultural conditions can not improve the hematopoietic recovery of post\|irradiated mice,but the expansion supported by bone marrow stromal cells can accelerate this process significantly.\;