经诱导的骨髓基质干细胞应用于组织工程化人工神经的实验研究

Experimental study of using induced bone marrow stromal cells into a tissue-engineered bioartificial nerve

目的 探讨骨髓基质干细胞应用于组织工程化人工神经修复大鼠 10mm长坐骨神经缺损的效果。 方法 2 8只体重在 160～ 2 0 0g的雌性F3 44大鼠随机分成 4组 ,每组 7只。A组 :种植经诱导 5d后的同源骨髓基质干细胞并具有内部支架结构的中空管 ;B组 :种植同源许旺细胞并具有内部支架结构的中空管 ;C组 :无细胞只具有内部支架结构的中空管 ;D组 :自体神经移植组。术后 3个月 ,进行系列神经电生理监测、坐骨神经功能指数测定、神经组织学观察、S 10 0及神经微丝蛋白免疫组化染色和轴突计数等检查。 结果 术后 12周内 ,实验组 (A组 )的各项检测指标均优于C组 (P <0 0 5或 0 0 1) ,与B和D组间差异无显著性 (P >0 0 5 )。 结论 初步结果显示经诱导的骨髓基质干细胞可作为外周神经组织工程中的种子细胞 ,并应用于人工神经修复外周神经缺损。

Objective To evaluate the effectiveness of using induced bone marrow stromal cells into a tissue-engineered bioartificial nerve on bridging a 10mm long sciatic nerve defect Methods Twenty-eight F344 female rats weighing 160～200 g were randomly divided into four groups of nerve grafting,with 7 rats in each group A group:PLGA tubes with an intrinsic framework were seeded with syngeneic bone marrow stromal cells induced 5 days;B group:PLGA tubes with an intrinsic framework were seeded with syngeneic Schwann cells;C group:PLGA tubes were only filled with an intrinsic framework;D group:autografts Three months later,a series of examinations were performed,including:electrophysiological methods,Walking trace's analysis,hisotological staining of nerves,S-100 and neurofilament immunostaining and axon counts Results At 12 weeks after the operations,all the examinations of A group were better than C group( P <0 05 or 0 01), But there were no statistically significant differences among A,B and D groups(P >0.05). Conclusions Induced bone marrow stromal cells as seeding cells for peripheral nerve tissue engineering were used to repair 10mm nerve gap,the outcome was satisfied.