甲壳质生物套管膜与许旺细胞生物相容性的实验研究

Experimental study of the biologic compatibility of the membrane of biologic conduit made of chitin and Schwann cells

目的 研究甲壳质生物套管膜与许旺细胞的生物相容性。 方法 将甲壳质生物套管膜与许旺细胞共同培养 ,观察、检测细胞在膜上和膜外的生长情况。 结果 在套管膜周围细胞形态和数量与对照培养孔内细胞无明显差别。套管膜上有细胞贴附生长。鼠尾胶原包被的套管膜上细胞数量较单纯套管膜上细胞数量多。 结论 甲壳质生物套管膜与许旺细胞有良好的生物相容性 ,经过鼠尾胶原包被后更有利于细胞贴附生长。

Objective Study the biologic compatibility of the membrane of biologic conduit made of chitin and Schwann cells in vitro Methods Cultured Schwann cells with the membrane,then observed their attachment,spread and growth Results Around the membrane,the shape,amounts and growth of Schwann cells in the experimental groups were same with the control group.Schwann cells can lived on the membrane,and their amounts were larger on the membrane precoated with collagen (rat tail) than on the simple membrane Conclusion The membrane of biologic conduit made of chitin have good biologic compatibility with Schwann cells and have better nerve cell affinity after being precoated with collagen.