马蹄金叶片离体培养及植株再生的研究

Plantlet Regeneration from Leaves in Vitro Culture of Dichondra repens Forst

在不同植物生长调节剂组合、不同培养基条件下,探讨了马蹄金叶片离体培养的方法和影响因素。结果表明,含不同植物生长调节剂组合的培养基对愈伤组织的诱导频率无显著影响,出愈率均达90%～100%;以MS或B5培养基作诱导愈伤组织的基本培养基效果较好;将叶片接种到MS+2mg/L6-BA的诱导培养基中诱导愈伤组织,再用B5+1.5mg/L6-BA+0.5mg/LZT+0.2mg/Lα-NAA作分化培养基出苗效果最佳,愈伤组织分化频率高达20%。

The methods and effect factors of plantlet regeneration from leaves in vitro culture of Dichondra repens Forst.were studied on different growth regulator combinations and different media.The results indicated that the kinds of growth regulator combinations had no significant effect on callus induction,the range of induction rate is 90% to 100%;It showed the optimal medium which could induce callus was MS medium and B5 medium;A mass of callus were induced from leaves in vitro culture on MS medium contained 2mg/L 6-BA,and then transferred to B5 medium supplemented with 1.5mg/L 6-BA,0.5mg/L ZT and 0.2mg/L α-NAA for regenerating,the differentiation rate is up to 20%.