聪圣胶囊对小鼠脑缺血损伤后细胞内游离钙含量的影响

Effect of Congsheng Capsule on Intracellular Calcium Concentration in Mice after Ischemic Cerebral Injury

目的 :研究聪圣胶囊对小鼠脑缺血再灌注后脑组织突触体及拟缺血损伤神经细胞内〔Ca2 + 〕i的影响 ,分析聪圣胶囊抗脑缺血损伤的作用机制。方法 :采用Fura 2 /AM双波长荧光分光光度法 ,检测小鼠脑组织突触体和胎鼠神经细胞悬液内〔Ca2 + 〕i。结果 :聪圣胶囊 3g/kg和 9g/kg灌胃给药可减轻小鼠脑缺血再灌注后突触体内钙超载程度 ;聪圣胶囊含药血清可抑制低糖低氧 1h及高浓度 (2 0 0 μmol/L)谷氨酸引起的神经细胞内钙的升高。结论 :聪圣胶囊可通过减轻脑缺血损伤后钙超载程度来发挥抗脑缺血作用。

Objective:To investigate the effect of Congsheng Capsule (CSC) in intracellular Ca 2+ concentration in cerebral intra-synaptosomes of ischemia/reperfusion mice and analogous ischemic injured neurons to estimate the mechanism of CSC in antagonizing ischemic cerebral injury. Methods:The concentration of Ca 2+ in cerebral intra-synaptosomes of mice and in neurocyte suspension of fetal mice were determined using Fura-2/AM double wavelength fluorescence spectrophotometer. Results:CSC 3 g/kg and 9 g/kg through gastrogavage could relieve the degree of calcium overloading of intra-synaptosomes in mice after cerebral ischemia/reperfusion. The CSC containing serum could inhibit the elevation of Ca 2+ content in neurocytes induced by 1 hour hypoxia and glutamate of high concentration (200μmol/L). Conclusion:CSC plays a role in antagonizing ischemic injury through relieving the calcium overloading after cerebral ischemia.