补肾方药对去卵巢大鼠主动脉晚期糖化终末产物及血脂的影响

Effect of Tonifying Shen Recipe on Advanced Glycosylation End Products in Aorta and Serum Lipids in Ovariectomized Rats

目的 :探讨补肾方药对去卵巢大鼠主动脉晚期糖化终末产物 (AGEs)、血脂和过氧化物的影响。方法 :将 6月龄雌性SD大鼠随机分为假手术组、去卵巢组和补肾方药组。大鼠去除双侧卵巢 2周后治疗 13周。禁食 2 4h ,取血处死动物 ,取主动脉 ,分别测定主动脉AGEs、血脂和血清过氧化物含量。结果 :各组大鼠总胆固醇 (TC)、载脂蛋白B(apoB)和低密度脂蛋白胆固醇 (LDL C)差异无显著性 (P >0 0 5 )。与假手术组比较 ,去卵巢组主动脉AGEs、甘油三酯 (TG)、氧化低密度脂蛋白 (OX LDL)、丙二醛 (MDA)均明显升高 (P<0 0 5或P <0 0 1) ;高密度脂蛋白胆固醇 (HDL C)、载脂蛋白A I(apoA I)和超氧化物歧化酶 (SOD)活性均显著降低 (均P <0 0 1)。与去卵巢组比较 ,补肾方药组主动脉AGEs、血清MDA和OX LDL均明显降低(P <0 0 5或P <0 0 1) ;HDL C、apoA I和SOD活性均显著升高 (均P <0 0 1)。结论 :补肾方药通过降低去卵巢大鼠主动脉AGEs含量 ,降低大鼠血清OX LDL和MDA水平 ,升高HDL C、apoA I水平和SOD活性 ,从而发挥其对心血管的保护作用。

Objective:To investigate the effect of tonifying Shen recipe (TSR) on levels of advanced glycosylation end products (AGEs) in aorta, serum lipids and lipid peroxidation in ovariectomized rats. Methods:Rats were randomly divided into the sham group, the ovariectomized group and the TSR group, in which the rats were treated with TSR for 13 weeks starting from 2 weeks after ovariectomy. Blood sample was taken out from rat at the end of the experiment after 24 hrs fasting for determination of lipids and lipid peroxidation, and the animal was sacrificed, the aorta was taken out for detecting AGEs. Results:No significant difference was found between groups in levels of total cholesterol and low density lipoprotein cholesterol. In comparing with the sham group, levels of aortic AGEs, serum triglyceride (TG), oxidized low density lipoprotein (OX-LDL) and malondialdehyde (MDA) in the ovariectomized group were obviously higher (P<0.05 or P<0.01), and levels of high density lipoprotein cholesterol (HDL-C), apo-lipoprotein A-I (apoA-I) and activity of superoxide dismutase (SOD) were lower (all P<0.01). While in the TSR group, as compared with the ovariectomized group, the above-mentioned abnormal changes, excepting for TG, were all reversed to certain degree (P<0.05 or P<0.01). Conclusion:TSR displays its cardiovascular protecting effect in ovariectomized rats through lowering the AGEs content in aorta, reducing the serum levels of OX-LDL and MDA, raising the levels of serum HDL-C and apoA-I and increasing SOD activity.